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. 2018 Apr 4;9:621. doi: 10.3389/fimmu.2018.00621

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Copyright © 2018 Saito, Suzuki, Yoshimoto, Kaneko, Yamaoka, Shimizu, Mori, Okamoto, Kameyama, Amano, Tamaru, Tokuhira and Takeuchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transition of proportion and absolute number of activated EMCD8+ T cell, mean fluorescence intensity (MFI) of HLA-DR on EMCD8+ T cell after methotrexate (MTX) cessation. Transition of (A) proportion and (B) absolute number of activated EMCD8+T cell, (C) MFI of HLA-DR onEMCD8+ T cell after MTX cessation. Comparison between the three groups was conducted by Kruskal–Wallis test, and comparison between two groups was conducted by Wilcoxon test. ^†Regressive vs. Control, P < 0.05. ^‡Regressive vs. Control, P < 0.05; Regressive vs. Persistent, P < 0.05. *P < 0.05 for the comparison with the value at week 0 in each group. EM, effector memory.