Figure 7.

swCDPK activity and protein in zygotic embryos and seedlings during seed maturation, dormancy, and germination. Proteins from zygotic embryo of MF and germination stages G1, G2, G3, and G4, and from shoot (Sh) of a 1-month-old plantlet were assayed for Ca²⁺-dependent phosphorylation of swCDPK protein, resolved on 10% (w/v) SDS-polyacrylamide gel, and transferred to nitrocellulose membrane. The proteins on this blot were then subjected to western-blot analysis using anti-soybean CDPK. A, Western blot; B, autophosphorylation of swCDPK. A and B represent the same blot. C, Western-blot analysis of proteins from zygotic embryos of stages RF, D, and G0. D, Autoradiogram of SDS-polyacrylamide gel depicting the autophosphorylation activity of 55-kD swCDPK in proteins of zygotic embryo of MF and G4, but not in zygotic embryo of stages RF, D, and G0. E, Graph representing Ca²⁺-dependent histone III-S phosphorylation activity in proteins of zygotic embryo/seedling from MF, RF, D, and germination stages G0, G1, G2, G3, and G4. The mean value of five replicates has been plotted. Black bars, 0.0 mm CaCl₂; white bars, 0.2 mm CaCl₂.