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. 2018 Apr 4;9:640. doi: 10.3389/fimmu.2018.00640

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Copyright © 2018 Nunes, Silva, Ampuero, Noronha, Souza, Correia, Khouri, Boaventura, Barral, Ramos, Brodskyn, Oliveira and Tavares.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of high confidence microRNAs is modulated in cutaneous lesions caused by Leishmania braziliensis. (A) The total number of probes, sequences, or high confidence microRNAs is presented for each data set, GSE55664 (purple) and GSE63931 (orange), from the outer to the inner circles, respectively (p < 0.05). (B) Bars graph shows the fold changes of high confidence microRNAs that differentially expressed from both datasets, GSE55664 (purple bars) and GSE63931 (orange bars), as well as real-time quantitative PCR to measure the relative expression of high confidence microRNAs in new samples from active lesions of patients with localized cutaneous leishmaniasis compared with healthy control skin for validation of the identified microRNAs in another cohort (black bars). The arrows indicate upregulated (red) or downregulated (green) expression (fold change ≥1.5).