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. 2018 Apr 4;9:612. doi: 10.3389/fimmu.2018.00612

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Copyright © 2018 Michels, van de Kar, Okrój, Blom, van Kraaij, Volokhina and van den Heuvel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Convertase activity in serum samples of healthy controls. Samples collected from 15 healthy controls were mixed 1:1 with pooled normal human serum (NHS) to an end concentration of 3.75%. Heat-inactivated NHS (ΔNHS) and an NHS sample from which guinea pig serum was omitted during the second part of the assay (no step 2) served as negative controls for the first and second steps, respectively. Means are given of ≥2 independent experiments for each control. Error bars were omitted for better visibility of the graph. Hemolysis levels are presented as percentage of full lysis of erythrocytes in water.