Genome reduction designs for M. florum L1. (A) Representation of four different versions of reduced M. florum L1 genome based on gene conservation, function, and essentiality. Genes are shown by a color representing core (C), noncore (NC), essential (E), or nonessential (NE) genes. In each case, the number of deleted bases is shown and corresponds to the sum of the lengths of the CDSs of the deleted genes. (B) Number of protein-encoding genes in each COG categories found in the different designs. The COG categories are as described in the legend to Fig. 2.