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. Author manuscript; available in PMC: 2019 Mar 21.
Published in final edited form as: Bioconjug Chem. 2017 Dec 27;29(3):604–612. doi: 10.1021/acs.bioconjchem.7b00692

Figure 3.

Figure 3

Self-assembled nanogels for toxoid delivery. (A) Schematic of a nano-sized hydrogel (cCHP nanogel) generated from cationic cholesteryl-bearing pullulan. (B) Quantitative study with [111In]-labelled Clostridium botulinum type-A neurotoxin subunit antigen Hc (BoHc/A) demonstrated that the antigen was retained in the nasal tissues significantly more when formulated using the cCHP nanogel. (C) Antibody-forming cells (AFCs) were effectively induced and recruited in the lamina propria and paranasal sinuses of the nasal mucosa 1 week after final immunization with cCHP–BoHc/A. (D) Strong C. botulinum type-A neurotoxin (BoNT/A)-specific IgA antibody responses were observed in nasal washes collected from mice intranasally immunized with cCHP–BoHc/A. (E) Strong BoNT/A-specific serum IgG antibody responses were induced by intranasal immunization with cCHP–BoHc/A. Reprinted with permission from ref 58. Copyright 2010 Springer Nature.