Figure 3.
Plasmids for sGFP fusion and promoter-GUS constructs. A, Five sGFP fusion plasmids with different sorts of putative localization signals (LS1, LS2, LS3, LS4, and LS6) fused to sGFP were constructed on pBI121. The numbers in the LS regions indicate amino acids lengths of signal peptide. 35S, Cauliflower mosaic virus 35S promoter; Tnos, nopaline synthase gene terminator. B, Five GGPP synthases genes promoters-GUS fusion plasmids were made. These plasmids had untranslated promoter regions of GGPS1 (0.9 kb), GGPS2 (1.7 kb), GGPS3 (2.0 kb), GGPS4 (2.0 kb), and GGPS6 (0.7 kb), which are transcriptionally fused by the GUS gene.