Figure 5.
In vitro import analysis of GGPS1 and GGPS3 proteins into pea chloroplast. Labeled proteins were made using rabbit reticulolysate and [35S]Met by in vitro translation. Lanes 1 to 3, GGPS1 protein; lanes 4 to 6, GGPS3 protein. Lanes 1 and 4 show the total in vitro-translated proteins of GGPS1 and GGPS3, respectively. Lanes 2 and 5 and lanes 3 and 6 show the labeled proteins after uptake by isolated pea chloroplasts with (lanes 3 and 6) or without (lanes 2 and 5) protease treatment. Arrowheads indicate the precursor (p) and mature (m) forms of those proteins. Molecular masses of the protein markers (kD) are indicated on the left.