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. 2018 Apr 4;9:275. doi: 10.3389/fphar.2018.00275

FIGURE 4.

FIGURE 4

miR-203 directly regulated the expression of Lipin1. (A) Bioinformatics analyses show the seed sequence of miR-203 bind to the 3′-UTR of LPIN1 mRNA. (B) “Wild type” 3′-UTR of LPIN1 gene was cloned into the firefly and Renilla reporter plasmid. The LPIN1-3′ UTR constructs or blank plasmid were transfected into AML-12 cells with control or miR-203 mimics, followed by dual luciferase assays. (C,D) Western blot analysis for protein expression of LPIN1 after transfected with miR-203 mimics or miR-NC, mir-203 inhibitor or inhibitor-NC. ∗p < 0.05, ∗∗p < 0.01 versus control group. #p < 0.05, ##p < 0.01 versus NC group.