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. 2018 Apr 4;9:275. doi: 10.3389/fphar.2018.00275

FIGURE 5.

FIGURE 5

Knocked down Lipinl contributed to inhibit hepatocyte lipids accumulation in vitro. (A) IHC analysis on liver tissues between the four groups. (B) Western blot analysis for Lipinl expression on mice liver tissues. (C) AML-12 cells were transfected with miR-203 mimic or miR-NC and then stimulated with ethanol 100 mM 24 h, Western analysis for Lipinl protein expression. (D) AML-12 cells were transfected with Lipinl-siRNA or control siRNA and then stimulated with ethanol 100 mM 24 h, Western analysis for Lipinl protein expression. (E) Western analysis for PPAR-α, SREBP-1, FASN protein expression after transfection. ∗p < 0.05, ∗∗p < 0.01 versus control group. #p < 0.05, ##p < 0.01 versus NC group.