Figure 3:
Comparison of two builds of gene annotation for the early chicken embryo samples. A) Using the Ensembl annotation with the two genome builds, annotated genes were compared based on the Ensembl ID. As a result, 5,657 and 14,651 Ensembl IDs were identified in Galgal4 and Galgal5, respectively, while 11,451 Ensembl IDs are common to the two different annotations. B) Based on the expressed genes at any stage of chicken early embryos, the gene lists were compared between Galgal4 and Galgal5. C) Investigation of the change in annotated genes in Galgal5 among genes expressed in early chicken embryos. As a result, a large number of lincRNAs was added as new features in Galgal5. D) A correlation analysis of the total gene expression based on 11,001 common annotated genes shared between Galgal4 and Galgal5.