Figure 4:

Multidimensional scaling plots based on all annotated genes in Galgal4 and Galgal5. The gene expression patterns of early chicken embryos quantified based on Galgal4 were clearly differentiated by developmental stage regardless of the sequencing technique used. In comparison, there was a difference between the bulked and single embryo sequencing techniques in the oocyte and zygote in Galgal5. The first dimension (Coordinate 1) is the progression of developmental stages in a negative direction during intrauterine development, and the second dimension (Coordinate 2) is the difference between oocyte and fertilized embryos from zygote to EGK.X.