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Flow-chart of study enrollment. Schematic overview of the recruitment, enrollment, and the follow-up of the study participants. At the start of the study, Dutch participants 25–29 years of age received a tetanus, diphtheria, and acellular pertussis booster vaccination. Blood samples were collected before (T0), 2 weeks (T1), 4 weeks (T2), 1 year (T3), and 2 years (T4) after the booster vaccination. *143 potential participants were excluded because the target for inclusion was achieved.
