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. 2018 Mar 22;74(Pt 4):214–221. doi: 10.1107/S2053230X18003242

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 96-well plates (VIOLAMO)
Temperature (K) 293
Protein concentration 0.10 mM (PCNA trimer; the protein solution includes 4.0 mM ZRANB3 APIM)
Buffer composition of protein solution 10 mM HEPES–NaOH pH 7.4, 100 mM NaCl
Composition of reservoir solution 0.2 M NaCl, 20% PEG 3350
Volume and ratio of drop 1:1 ratio protein:reservoir (1 µl)
Volume of reservoir (µl) 40