Table 2. Crystallization.
Method | Sitting-drop vapour diffusion |
Plate type | 96-well plates (VIOLAMO) |
Temperature (K) | 293 |
Protein concentration | 0.10 mM (PCNA trimer; the protein solution includes 4.0 mM ZRANB3 APIM) |
Buffer composition of protein solution | 10 mM HEPES–NaOH pH 7.4, 100 mM NaCl |
Composition of reservoir solution | 0.2 M NaCl, 20% PEG 3350 |
Volume and ratio of drop | 1:1 ratio protein:reservoir (1 µl) |
Volume of reservoir (µl) | 40 |