Figure 3.

Physical association between the α-CT and β-CT subunits of the heteromeric ACCase. Preimmune or anti-CAC3 serum was incubated with Arabidopsis leaf extracts. Antibody-antigen complexes were bound to protein A-agarose and collected by centrifugation. The resulting pellets were boiled in the presence of 2% (w/v) SDS and, following centrifugation, aliquots of the supernatants were subjected to western-blot analyses. These blots were sequentially probed with anti-BCC, anti-BCase, anti-α-CT, and anti-β-CT sera. The positions of the BCC, BCase, α-CT, and β-CT polypeptides are indicated by asterisks (*).