Skip to main content
. 2018 Mar 22;74(Pt 4):187–192. doi: 10.1107/S2053230X18002868

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type Rigaku Reagents XJR
Temperature (K) 290
Protein concentration (mg ml−1) 22.4
Buffer composition of protein solution 300 mM NaCl, 20 mM HEPES, 5% glycerol, 1 mM TCEP pH 7.0
Composition of reservoir solution 200 mM ammonium formate pH 6.6, 20% PEG 3350
Protein:precipitant 0.4 µl:0.4 µl
Volume of reservoir (µl) 80