Table 2. Crystallization.
Method | Vapor diffusion, sitting drop |
Plate type | Rigaku Reagents XJR |
Temperature (K) | 290 |
Protein concentration (mg ml−1) | 22.4 |
Buffer composition of protein solution | 300 mM NaCl, 20 mM HEPES, 5% glycerol, 1 mM TCEP pH 7.0 |
Composition of reservoir solution | 200 mM ammonium formate pH 6.6, 20% PEG 3350 |
Protein:precipitant | 0.4 µl:0.4 µl |
Volume of reservoir (µl) | 80 |