Table 1. Macromolecule production.

The BamHI site and the EcoRI site are underlined. The tag residues removed by PreScission protease are shown in italics.

Source organism	P. aeruginosa PAO1
DNA source	Genome
Forward primer	CGCGGATCCATGAGTTTCAGCCCGCTGATCC
Reverse primer	CCGGAATTCTCAGGAGATCGGCCGCCGTCCG
Cloning vector	pGEX-6P-1
Expression vector	pGEX-6p-1
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	GPLGSMSFSPLIRQLIESLRILPGVGQKSAQRMALMLLERDRSGGLKLAQALTAAMEGVGHCRQCRTLSEEELCPQCADPRRDDSLLCVVEGPLDVFAVEQTGYRGRYFVLKGHLSPLDGLGPEAIGIPELEARIRDGAFSEVILATNPTVEGEATAHYIAQLLAGRGLTLSRIAHGVPLGGELELVDGGTLAHALAGRRPIS