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. 2018 Apr 10;11:233. doi: 10.1186/s13071-018-2699-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Limit of detection of T. cruzi in açai samples with primers Tc189F/Tc189R. T. cruzi DNA (from 75 pg to 2.4 pg) was mixed with 240 ng of açai DNA before the PCR assay using Tc189F/189R primers. Lane 1: sterile water (negative control); Lane 2: T. rangeli DNA (5 ng); Lane 3: Açai without T. cruzi (negative control); Lane 4: 100 bp DNA ladder. T. cruzi DNA: Lane 5: 75 pg; Lane 6: 45 pg; Lane 7: 24 pg; Lane 8: 15 pg; Lane 9: 9 pg; Lane 10: 3 pg; Lane 11: 2.4 pg