Fig. 5.

Loops A and B are required for specific and robust FAMA stage MKK7 activities. a–f Paired micrographs of representative major phenotype (left) and subcellular localization (right), of specific MKK7^ED-YFP variants diagramed above. Black brackets mark clusters and asterisks indicate inhibition. YFP is in green and cell outlines in magenta; white arrowheads point to mitochondrial localization. For example, in (a) MKK7^ED induces stomatal clustering (brackets) and is mitochondrial localized (white arrowheads). Scale bars are 50 μm for phenotype images and 10 μm for localization images. g Quantification of phenotypes in (a–f). h SPCH stage inhibition of lineage initiation. Error bars in (g) and (h) correspond to 95% confidence interval. i In vitro kinase activity towards kinase inactive MPK3 and MPK6. Kinase assays were performed in triplicates, normalized to unphosphorylated KI-MPK and averaged; error bars represent standard errors