Table 1.
Quantification of in vivo and in vitro phenotypes conferred by chimeras
| Name | Kinase Activity (normalized to MKK5DD) | SPCH stage inhibition of linage initiation | FAMA stage phenotypes (as percentage of total T1 seedlings) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KI-MPK3 | KI-MPK6 | n | % | n | Inhibited | WT | GC clusters | |||||
| AVG | SE | AVG | SE | Small | Large | Total | ||||||
| YFP control | 24 | 0.00 | 91.67 | 8.33 | 0.00 | 8.33 | ||||||
| MKK5DD | 34.485 | 9.052 | 38.259* | 2.308 | 69 | 78.26 | 25 | 0.00 | 76.00 | 24.00 | 0.00 | 24.00 |
| MKK7ED | 6.836 | 4.808 | 10.867 | 5.726 | 93 | 100.00 | 50 | 20.00 | 26.00 | 0.00 | 54.00 | 54.00 |
| N7-MKK5DD | 5.500 | 1.894 | 21.448 | 9.176 | 75 | 26.67 | 125 | 8.80 | 32.00 | 48.80 | 10.40 | 59.20 |
| N5-MKK7ED | 15.889 | 7.052 | 35.235* | 1.457 | 22 | 86.36 | 47 | 93.62 | 6.38 | 0.00 | 0.00 | 0.00 |
| MKK5DD-C7 | 13.888 | 9.753 | 25.666* | 2.530 | 47 | 87.23 | 44 | 65.91 | 29.55 | 4.55 | 0.00 | 4.55 |
| MKK5DD-7A | 45.980 | 22.674 | 26.287 | 4.947 | 114 | 12.28 | 98 | 0.00 | 97.96 | 2.04 | 0.00 | 2.04 |
| MKK5DD-7B | 46.746 | 22.671 | 38.718* | 0.750 | 20 | 10.00 | 70 | 50.00 | 50.00 | 0.00 | 0.00 | 0.00 |
| MKK7ED-C5 | 3.877 | 2.091 | 0.728 | 0.148 | 43 | 13.95 | 19 | 0.00 | 78.95 | 21.05 | 0.00 | 21.05 |
| MKK7ED-CDR5 | 2.451 | 1.242 | 1.303* | 0.312 | 89 | 98.81 | 30 | 96.67 | 3.33 | 0.00 | 0.00 | 0.00 |
| MKK7ED-CPR5 | 15.844 | 11.444 | 5.904 | 2.422 | 105 | 0.00 | 36 | 2.78 | 91.67 | 5.56 | 0.00 | 5.56 |
| MKK7ED-5A | 15.440 | 12.606 | 5.219 | 4.017 | 125 | 88.00 | 54 | 81.48 | 14.81 | 3.70 | 0.00 | 3.70 |
| MKK7ED-5B | 7.238 | 4.545 | 1.317 | 0.200 | 198 | 16.67 | 60 | 0.00 | 48.33 | 40.00 | 11.67 | 51.67 |
| N7-MKK5DD-C7 | 3.526 | 1.153 | 2.411 | 0.915 | 91 | 54.95 | 43 | 2.33 | 13.95 | 13.95 | 69.77 | 83.72 |
| N5-MKK7ED-C5 | 4.724 | 2.876 | 1.439 | 0.673 | 51 | 72.55 | 46 | 0.00 | 71.74 | 28.26 | 0.00 | 28.26 |
| MPK3, not shown | 49 | 0.00 | 50 | 0.00 | 82.00 | 18.00 | 0.00 | 18.00 | ||||
| MPK3D193G/E197A | 18 | 0.00 | 52 | 0.00 | 80.77 | 19.23 | 0.00 | 19.23 | ||||
| MPK3T119C, not shown | 43 | 0.00 | 89 | 0.00 | 86.52 | 13.48 | 0.00 | 13.48 | ||||
| MPK6, not shown | 122 | 0.00 | 45 | 0.00 | 84.44 | 15.56 | 0.00 | 15.56 | ||||
| MPK6D218G/E222A | 6 | 100.00 | 27 | 0.00 | 77.78 | 22.22 | 0.00 | 22.22 | ||||
| MPK6Y144C, not shown | 35 | 0.00 | 21 | 0.00 | 90.48 | 9.52 | 0.00 | 9.52 | ||||
Kinase activity is determined in triplicates with the exception of those marked with “*” that were done in duplicates. Averages (AVG) and Standard Errors (SE) are represented in the table. Phenotypic categories for FAMA stage phenotypes are described in Methods