Table 2.
Expression analysis of select genes
| Paraquat treatment compared to water |
BdbZIP10-GFP OE Compared to WT |
Putative function Phytozome/Panther |
|||
|---|---|---|---|---|---|
| Gene Name | BdbZIP10-GFP OE | WT | Water | Paraquat | |
| Bradi2g33110.1 | 0.2–0.48 | 0.24–0.33 | 2.0–4.1 | 2.1–4.9 | Bradi2g33110.1.p - zinc ion transmembrane transporter activity |
| Bradi2g22520.1 | 0.28–0.62 | 2.1–2.8 | 52.8–93.1 | 8.1–18.2 | Zinc ion transmembrane transporter activity |
| Bradi1g53680.1 | 0.05–0.14 | 0.24–1.17 | 30–123 | 5.4–18.1 |
High affinity zinc uptake
transmembrane transporter activity |
| Bradi3g17900.1 | 0.27–0.68 | 0.6–1.2 | 6.4–14.8 | 3.1–7.8 |
Zinc ion transmembrane
transporter activity |
| Bradi5g21580.1 | 0.16–0.25 | 0.8–1.2 | 1.1–2.4 | 1.0–1.4 | High affinity zinc uptake transmembrane transporter activity |
| Bradi1g68300 | 3.9–5.2 | 3.9–5.1 | 0.8–1.2 | 0.9–1.3 | PTHR23310:SF14-Protein ACBP-7 |
| Bradi1g37100 | 0.59–0.81 | 0.72–1.63 | 2.7–5.9 | 2.1–3.0 | PF01152 – Bacterial-like globin; Thoredoxin peroxidase |
| Bradi1g49020 | 0.4–0.6 | 3.3–7.4 | 3.4–8.0 | 0.4–0.6 | PTHR14209//PTHR14209:SF7- Isoamyl acetate-hydrolyzing esterase 1 |
| Bradi3g41600 | 3.9–5.2 | 7.8–13.1 | 1.2–1.7 | 0.5–0.7 | Stress up-regulated Nod19 (SURNod19) |
Comparison of gene expression levels between paraquat and water treated BdbZIP10 OE and wildtype plants. Gene expression level of BdbZIP10-GFP OE compared to wildtype plants. Numbers indicate the range of three independently BdbZIP10-GFP OE transformed lines, calculated using the standard deviation of the Ct values. Bold text indicates the most significant changes between BdbZIP10-GFP OE and wildtype plants