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. 2018 Apr 10;15:31. doi: 10.1186/s12977-018-0414-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — A3Z3 sensitivity of the felids related to pumas and bobcats to PLV Vif-mediated degradation. HA-tagged expression plasmids for cheetah (a, b) and lynx (c, d) A3Z3 (200 ng) and the three plasmids used to produce the vif-deficient FIV-based reporter virus (FIV plasmids; pFP93 [200 ng], pTiger-luc [150 ng] and pMD.G [50 ng]) were co-transfected with or without His-tagged PLV Vif expression plasmids (400 ng) into HEK293T cells. a, c Western blotting. Representative results are shown. b, d FIV reporter assay. FIV infectivity is shown as the percentage of the value of “no A3Z3”. In b and d, asterisks indicate significant differences (P < 0.05 by Student’s t test) versus “no Vif”. The assays were independently performed in triplicate. Data represent averages with SDs