Fig. 5.

Loss-of-function screening of the amino acid residues responsible for PLV-B Vif sensitivity. HA-tagged expression plasmids for the A3Z3 derivatives (indicated in the figure) of the bobcat (200 ng) and the three plasmids used to produce the vif-deficient FIV-based reporter virus (FIV plasmids; pFP93 [200 ng], pTiger-luc [150 ng] and pMD.G [50 ng]) were co-transfected with or without His-tagged PLV-B Vif expression plasmids (400 ng) into HEK293T cells. a, c Western blotting. Representative results are shown. b, d FIV reporter assay. FIV infectivity is shown as the percentage of the value of “no A3Z3”. In b and d, asterisks indicate significant differences (P < 0.05 by Student’s t test) versus “no Vif”. The assays were independently performed in triplicate. Data represent averages with SDs