The effects of recombinant human Netrin-1 (rh-NTN-1) and knockdown of endogenous NTN-1 on expression of inflammatory molecules at 24 h after SAH. Exogenous rh-NTN-1 treatment further augmented PPARγ expression, and inhibited the expression of NFκB, TNF-α, IL-6, and ICAM-1 in the ipsilateral cortex (A–F). In contrast, when depleted the endogenous NTN-1 with specific siRNA, PPARγ expression was decreased, but the expression of NFκB, TNF-α, IL-6, and ICAM-1 were increased (A–F). Relative densities of each protein have been normalized against the sham group. n=6/group. *P<0.05 vs sham; #P<0.05 vs PBS; and &P<0.05 vs PBS, rh-NTN-1 and Scr siRNA. NTN-1 siRNA, Netrin-1 siRNA; Scr siRNA, scrambled siRNA.