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. 2018 Apr 4;5:87. doi: 10.3389/fmed.2018.00087

Figure 2.

Figure 2

A rational approach to design of transcriptomics study. An overview of steps to help researchers make appropriate study design is presented. First, distinguish whether study aims to identify biomarkers or mechanisms. Then, the source of tissues together with power analysis to calculate sample size to be able to answer research question should be performed. Decision about the type of analysis should be made (bulk, sorted cells, or single cells). Last, the technology to perform transcriptome analysis should be chosen.