Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 4;6:33. doi: 10.3389/fcell.2018.00033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Gojanovich, Gimenez, Masone, Rodriguez, Dewey, Delgui, Bustos and Uhart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Isolation of mesenchymal stem/stromal cells from human solid adipose tissue. (A) solid white adipose tissue samples are received in 120 mL disposable sterile plastic containers, (B) the samples are placed on a Petri dish before starting their dissection, (C) fat is separated from skin (which is discarded), (D) fat tissue is cut with scissors, (E) fat is cut further with surgical blades, (F) fat is rinsed with PBS buffer in polypropylene conical tubes, (G) obtaining of three phases after filtration and centrifugation, (H) separation of the stromal vascular fraction (SVF) from the other two phases, (I) seeding the washed SVF in 10 cm diameter plate with supplemented DMEM.