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. 2018 Apr 4;6:33. doi: 10.3389/fcell.2018.00033

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Copyright © 2018 Gojanovich, Gimenez, Masone, Rodriguez, Dewey, Delgui, Bustos and Uhart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid droplets and cellular proteins double staining. (A) by Untreated (UT) and differentiated (D) hASCs were Oil Red O (ORO) and immunostained, and analyzed by fluorescence microscopy. Green signal corresponds to the indicated proteins detected by immunofluorescence (Actin-FITC) or indirect immunofluorescence (EEA-1, GM 130, ADRP); blue signal corresponds to Hoechst-stained nuclei; red signal corresponds to ORO-stained LDs. (B) Automated quantification of ORO fluorescence intensity relative units (R.U.) in UT and D hASCs. Bars represent mean values (+/– standard deviation) from 14 ORO-stained images prepared as in A and normalized to the number of nuclei/image. The asterisk indicates significant differences between samples (Student's test; p-value < 0.01). Data were similar in two other experiments.