Figure 5.

SDS-PAGE and Western blot analysis of differentiated and control hASCs. Proteins from whole cell lysates of untreated (UT) or differentiated (D) hASCs were solved in 12% acrylamide gels and stained with Coomasie blue (left image). The proteins were transferred to PVDF membranes from an unstained 12% acrylamide gel and stained with Ponceau S (central image). Immune anti-β-actin detection in hASCs whole cell lysates. 10 μg of whole cell lysate were loaded/lane. A representative image from three experiments is shown.