Component	Volume (mL)	Final concentration
1 M Tris-HCl pH 6.8	0.320	0.13 M
30% Acrylamide/0.8% Bisacrylamide	0.620	7.4%/0.2%
20% SDS	0.0125	0.10%
TEMED	0.0025	0.10%
10% APS	0.025	0.10%
H2O	1.5	–