| Component | Volume (mL) | Final concentration |
|---|---|---|
| Glycerol | 1 | 13.40% |
| 1.5 M Tris-HCl, pH 8.8 | 1.9 | 0.38 M |
| 30% Acrylamide/0.8% Bisacrylamide | 3 | 12%/0.30% |
| 20% SDS | 0.037 | 0.10% |
| TEMED | 0.003 | 0.04% |
| 10% APS | 0.037 | 0.05% |
| H2O | 1.45 | – |
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