Table 2.

Inhibition of canonical NF-κB signalling by simplified helenalin analogues. Values are the mean ± S.D. from n ≥ 3 biological replicates and were normalized to a no compound, induced control.

no Michael acceptors	NF-κB activity (%; Mean ± S.D.)
	250 µM	100 µM
9	86 ± 1	91 ± 3
10	86 ± 5	97 ± 8
13	78 ± 5	84 ± 1
15	99 ± 8	99 ± 7
16	79 ± 6	82 ± 2
enone only	250 µM	100 µM	50 µM
11	50 ± 5	85 ± 3	92 ± 6
12	34 ± 4	69 ± 4	93 ± 11
14	43 ± 4	73 ± 3	-
17	78 ± 9	86 ± 12	95 ± 5
18	63 ± 4	83 ± 8	88 ± 6
S4	23 ± 4	62 ± 2	-
butyrolactone only	50 µM	20 µM	10 µM
19	11 ± 2	43 ± 1	71 ± 2
20	12 ± 1	42 ± 2	69 ± 2
1b[a]	7 ± 1	16 ± 8	52 ± 17

^[a]Compound 6b from ref. 9b. 10 µM data reported previously.^[9b]