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. 2018 Apr 1;24:1912–1923. doi: 10.12659/MSM.905833

Figure 6.

Figure 6

SphK1 leads to increase S1P levels and enhances Notch signaling pathway via S1PR3. (A) TNBC cells MDA-MB-231 were transfected with Ad-NC-siRNA or Ad-SPHK1-siRNA #2 at 10 MOI for 24 hours, RT-PCR assay was using for detection the expression of Hes1 (Notch signaling target gene), Gli1 (Hedgehog signaling target gene), and Dkk1 (Wnt signaling target gene). (B) MDA-MB-231 cells were treatment with S1P (200 nM) or LPA (200 nM) for 24 hours, expression levels of Hes1, Gli1, and Dkk1 were detected by RT-PCR. (C) Effects of TY52156 (2 mM), CAY10444 (5 mM) or JTE013 (2 mM) on S1P-induced Hes1 expression by RT-PCR. Data represent mean ±SD (n=3). (D) Effects of S1P, LPA, and N1ICD overexpression (N1ICD OE) on N1ICD production by western blotting, GAPDH was regard as control.