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. 2000 Apr;122(4):1129–1136. doi: 10.1104/pp.122.4.1129

Figure 1.

Figure 1

Mutation site of P5CS that removed feedback inhibition by Pro, and restriction map of pBI-P5CSF129A. Codon TTT at nucleotide positions 421 to 423 of the V. aconitifolia P5CS cDNA (Hu et al., 1992) was changed to GCC by site-directed mutagenesis so that Phe (F) at amino acid position 129 of the P5CS peptide is replaced by Ala (A), generating P5CSF129A. The mutant enzyme retains similar kinetic characteristics as the wild-type P5CS, except that its allosteric regulation by Pro is eliminated (Zhang et al., 1995).