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. 2018 Apr 4;11:1871–1884. doi: 10.2147/OTT.S157747

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© 2018 Jing et al. This work is published and licensed by Dove Medical Press Limited

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AA-PMe inhibits VEGFR2 activation in zebrafish embryos. (A) Total and phospho-VEGFR2 expression in 72 hpf whole embryo lysates following treatment with the indicated doses of AA-PMe or AA. A representative blot is shown. (B) Ratio of phospho to total VEGFR2 expression as determined by the densitometric analysis. Bars represent the mean ± SEM from three separate experiments. *P<0.05, **P<0.01.

Abbreviations: AA, asiatic acid; AA-PMe, N-(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; HUVEC, human umbilical vein endothelial cell; VEGFR2, vascular endothelial growth factor receptor 2.