Figure 1.
A, Predicted amino acid sequences of snapdragon cyclin D1, cyclin D3a (Am-CycD3a), and cyclin D3b (Am-CycD3b), illustrating conserved (black) and similar (gray) residues with D-cyclins from Arabidopsis (At-CycD1, 2, and 3; Soni et al., 1995). The extent of the cyclin box is indicated by two asterisks. Dots indicate gaps introduced to maximize sequence alignment. The retinoblastoma interaction motif is underlined. EMBL accession numbers are cyclin D1, AJ250396; cyclin D3a, AJ250397; and cyclin D3b, AJ250398. B, Southern analysis of snapdragon genomic DNA cut with HindIII (H), BamHI (B), and EcoRI (E) and probed with cyclin D1 (D1), cyclin D3a (D3a), and cyclin D3b (D3b). The positions of the 2-, 3-, 4-, and 10-kb molecular mass markers are indicated by bars.