Comparison of matched unstained (a, c) and picrosirius red stained (b, d) sections of a superficial digital flexor tendon, SDFT (a, b) and a common extensor tendon, CDET (c, d). The false-coloured images encode the phase shift |sin δ| which is proportional to birefringence (see Eq. (1). Higher birefringence is observed in stained sections (b and d), as compared to the unstained sections (a and c). Scale bars = 100 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)