Figure 1.

Identification of CLC-Interacting Proteins.

(A) TAP-MS-based isolation of proteins associated with clathrin. CLC1 in C- and N-terminal fusions with TAP tags was used as bait. Two repetitions with each construct were performed.

(B) BiFC-based interactions between CLC1 and selected interactors identified with TAP-MS. Pairs of proteins of interest fused with halves of the GFP molecule were transiently expressed in wild tobacco leaves and reconstituted GFP fluorescence was imaged in epidermis on the abaxial leaf side. PIP5K1 did not interact with any of the proteins tested and was used as a negative control.

(C) Schematic representation of all interactions detected with BiFC. Additional interactions are presented in Supplemental Figure 1.