Figure 4.

Inhibition of FM4-64 Endocytic Tracer Uptake by AUXILIN-LIKE1/2 Overexpression.

(A) The uptake of the endocytotic tracer FM4-64 into RAM epidermal cells in XVE»AUXILIN-LIKE1/2 seedlings induced for 16 to 24 h is strongly reduced. At least 10 roots per genotype were imaged in three repeated experiments. The graphs shows quantification of intracellular-to-PM FM4-64 signal ratio from 40 cells coming from four roots in a representative experiment.

(B) FM4-64 uptake at early stages (8 h) of INTAM»GFP-AUXILIN-LIKE1 induction. The degree of FM4-64 uptake correlated with GFP-AUXILIN-LIKE1 levels in individual root meristem epidermal cells. Graphs show examples of this correlation in four roots. Each data point represents an individual cell. Notice that at this stage of AUXILIN-LIKE1 overexpression, cell morphology remains normal, with abundant cytosol illuminated by GFP-AUXILIN-LIKE1 expression, and small, fragmented vacuoles seen by negative staining. a.u., arbitrary units.