Figure 7.

Increased PM Binding of Endocytic Adaptor Protein Complexes in XVE»AUXILIN-LIKE1 Line.

Overexpression of AUXILIN-LIKE1 caused increased PM binding of TPLATE, a subunit of the TPLATE complex, as well as AP2A1, a subunit of the AP-2 complex. CLSM images from RAM epidermis ([A] and [B]) and VAEM images from hypocotyl epidermis ([C] and [D]) are shown. Graphs in (A) and (B) show PM signal intensity measurements from a representative experiment. Kymographs ([E] and [F]) show reduced lifetimes of TPLATE and AP2A1 foci in XVE»AUXILIN-LIKE1 line, likely indicating that endocytic pits aborted after initiation. All experiments in this figure were performed after 16 to 24 h of XVE»AUXILIN-LIKE1 induction.