Figure 8.

Excess Membrane Accumulation at the PM as a Result of Inhibited Endocytosis.

Overexpression of AUXILIN-LIKE2 caused excess deposition of membrane material at the PMs of root epidermis, as visualized with FM4-64 staining ([A] and [B]) or with PIN2-GFP (C). (A) and (C) are maximum intensity projections of z-stacks through outer region of the epidermis, while (B) is a longitudinal section of epidermal cells showing membrane aggregation mainly in the outer domain. FM4-64-stained deposits in Col-0 in (A) are likely remnants of apoptosed lateral root cap cells.