Figure 3.

Proteomic Analysis of the MS-Identified Arabidopsis Myristoylome.

The Arabidopsis genome contains 2587 predicted open reading frames with a Gly at position 2 (MGxx set). Within those, 906 proteins were detected by MS (10% of the starting candidate pool of 8837 MS-detected proteins); 214 of these were part of the predicted myristoylome and 692 were not. Of the 214 in the predicted myristoylome, the myristoyl moiety was detected for 54 via targeted MS, 159 lacked identification of the N-terminal peptide, and only one had an N-acetylated or free N terminus. The 207 remaining predicted MYRed proteins were not detected in the MS-identified set. Within the set of 692 MS-identified proteins not predicted to be MYRed (but carrying a Gly2, i.e., in the MGxx set), 18 had a MYRed peptide detected via targeted MS and 50 had an acetyl or free N terminus.