Table 1. Overview of Identified MYRed Proteins Recovered with the N-Terminal MYRed Peptide.
| Accessiona | Protein Nameb | Clusterc | Pred. Myristoylomed | Observed MYRed Peptidee |
N-Terminal Protein Sequencef | Myristoyl Moiety Identified on Peptideg | MYR-Gly Positionh | |
|---|---|---|---|---|---|---|---|---|
| LTQ-Orbitrap | Triple-Quad | |||||||
| AT3G09540.1 | Pectin lyase-like | IV.3.a | X | X | X | MGNLHGIHRS | C14-gNLHGIHR | 2 |
| AT4G38690.1 | 1-Phosphatidylinositol phosphodiesterase-like | IV.1.b | X | X | MGSHLSKQLE | C14-gSHLSK | 2 | |
| AT4G25970.1 | Phosphatidylserine decarboxylase (PSD3) | IV.1.b | X | X | MGNGNSTETK | C14-gNGNSTETK | 2 | |
| AT5G51570.1 | Hypersensitive induced reaction (HIR3) | III.1.a | X | X | MGNTYCILGG | C14-gNTYcILGGcIEQASVGVVER | 2 | |
| AT5G62740.1 | Hypersensitive induced reaction (HIR4) | IV.1.a | X | X | MGNLFCCVQV | C14-gNLFccVQVDQSTVAIK | 2 | |
| AT1G69840.1 | Hypersensitive induced reaction (HIR1) | IV.1.b | X | MGQALGCIQV | C14-gQALGcIQVDQSNVAIK | 2 | ||
| AT3G01290.1 | Hypersensitive induced reaction (HIR2) | IV.3.a | X | X | MGNLFCCVLV | C14-gNLFccVLVK | 2 | |
| AT5G63030.1 | Glutaredoxin-C1 | III.1.a | X | X | X | MGSMFSGNRM | C14-gSmFSGNR | 2 |
| AT2G48150.1 | Glutathione peroxidase 4 (GPX4) | IV.2 | X | X | X | MGASASVPER | C14-gASASVPER | 2 |
| AT3G63080.1 | Glutathione peroxidase 5 (GPX5) | IV.1.a | X | X | X | MGASSSSSVS | C14-gASSSSSVSEK | 2 |
| AT1G69880.1 | Thioredoxin H-type 8 (TrxH8) | X | X | MGANVSTPDQ | C14-gANVSTPDQR | 2 | ||
| AT3G08710.1 | Thioredoxin H-type 9 (TrxH9) | IV.1.b | X | X | MGSCVSKGKG | C14-gSCVSK | 2 | |
| AT5G06260.1 | TLD-domain containing nucleolar protein | X | X | MGNSNSSSVD | C14-gNSNSSSVDHR | 2 | ||
| AT2G45380.1 | TLD-domain unknown function | X | K.GNSNSSSVDHR.F | C14-gNSNSSSVDHR | 98 | |||
| AT1G70490.1, AT3G62290.1, AT1G10630.1 | ADP-ribosylation factor (ARFA1D); (ARFA1E); (ARFA1F) | II.1 | X | X | MGLSFAKLFS | C14-gLSFAKLFSRLFAK | 2 | |
| X | X | MGLSFGKLFS | C14-gLSFGKLFSKLFAK | 2 | ||||
| X | X | MGLSFAKLFS | C14-gLSFAK | 2 | ||||
| AT4G35230.1 | IV.1.b | X | X | MGCCQSLFSG | C14-gccQSLFSGDNPLGK | 2 | ||
| AT5G46570.1 | BR-signaling kinase 2 (BSK2) | IV.1.b | X | X | X | MGCLHSKTAN | C14-gcLHSK | 2 |
| AT4G00710.1 | BR-signaling kinase 3 (BSK3) | IV.1.b | X | X | X | MGGQCSSLSC | C14-gGQcSSLSccR | 2 |
| AT5G41260.1 | BR-signaling kinase 8 (BSK8) | IV.1.b | X | X | MGCEVSKLSA | C14-gCEVSK (possible ambiguity) | 2 | |
| AT1G63500.1 | BR-signaling kinase 7 (BSK7) | IV.1.b | X | X | MGCEVSKLCA | 2 | ||
| AT3G06270.1 | Protein phosphatase 2C family protein (PP2C -like) | IV.1.b | X | X | MGCVQCKCCS | C14-gcVQcK | 2 | |
| AT1G03590.1 | Protein phosphatase 2C family protein (PP2C6) | IV.1.b | X | MGGCISKTSW | C14-gGCISK | 2 | ||
| AT1G16220.1 | Protein phosphatase 2C family protein (PP2C -like) | X | X | MGLCHSKIDK | C14-gLCHSK | 2 | ||
| AT4G03415.1 | Protein phosphatase PP2C52 | IV.1.b | X | X | MGGCVSTSSK | C14-gGcVSTSSK | 2 | |
| AT1G14370.1 | Protein kinase (KIN1) | IV.1.b | X | MGNCLDSSAK | C14-gNcLDSSAK | 2 | ||
| AT2G02800.1 | Protein kinase (KIN2) | IV.3.b | X | MGNCLDSSAK | C14-gNCLDSSAK | 2 | ||
| AT5G11410.1 | Protein kinase superfamily | X | MGNCLKPLKE | C14-gNcLKPLK | 2 | |||
| AT2G20140.1, AT4G29040.1 | 26S protease regulatory complex subunit (RPT2b, RPT2a) | IV.3.b | X | X | X | MGQGPSGGLN | C14-gQGPSGGLNR | 2 |
| X | X | MGQGPSGGLN | C14-gQGPSGGLNR | 2 | ||||
| AT3G09770.1 | Ubiquitin E3 ligase (LOG2) | X | X | MGNISSSGGE | C14-gNISSSGGEGR | 2 | ||
| AT4G17615.1 | Calcineurin B-like protein 1 (CBL1) | IV.1.b | X | X | MGCFHSKAAK | C14-gCFHSK | 2 | |
| AT5G47100.1 | Calcineurin B-like protein 9 (CBL9) | IV.1.b | X | X | X | MGCFHSTAAR | C14-gcFHSTAAR | 2 |
| AT5G04870.1 | Calcium-dependent protein kinase 1 (CPK1) | IV.1.b | X | X | X | MGNTCVGPSR | C14-gNTcVGPSR | 2 |
| AT4G35310.1 | Calcium-dependent protein kinase 5 (CPK5) | IV.1.b | X | X | MGNSCRGSFK | C14-gNSCR | 2 | |
| AT2G17290.1 | Calcium-dependent protein kinase 6 (CPK6) | IV.1.b | X | X | MGNSCRGSFK | C14-gNSCR | 2 | |
| AT5G12480.1 | Calcium-dependent protein kinase 7 (CPK7) | IV.1.b | X | X | X | MGNCCGNPSS | C14-gNccGNPSSATNQSK | 2 |
| AT5G19450.1 | Calcium-dependent protein kinase 8 (CPK8) | IV.1.b | X | X | X | MGNCCASPGS | C14-gNccASPGSETGSK | 2 |
| AT3G20410.1 | Calcium-dependent protein kinase 9 (CPK9) | IV.1.b | X | X | X | MGNCFAKNHG | C14-gNcFAK | 2 |
| AT1G18890.1 | Calcium-dependent protein kinase 10 (CPK10) | IV.1.b | X | X | X | MGNCNACVRP | C14-gNcNAcVRPDSK | 2 |
| AT3G51850.1 | Calcium-dependent protein kinase 13 (CPK13) | IV.1.b | X | X | MGNCCRSPAA | C14-gNCCR | 2 | |
| AT4G21940.1 | Calcium-dependent protein kinase 15 (CPK15) | IV.1.b | X | X | MGCFSSKHRN | C14-gCFSSK | 2 | |
| AT4G04720.1 | Calcium-dependent protein kinase 21 (CPK21) | IV.1.b | X | X | MGCFSSKHRK | C14-gCFSSK | 2 | |
| AT1G74740.1 | Calcium-dependent protein kinase 30 (CPK30) | IV.1.b | X | X | X | MGNCIACVKF | C14-gNcIAcVK | 2 |
| AT3G57530.1 | Calcium-dependent protein kinase 32 (CPK32) | IV.1.b | X | X | X | MGNCCGTAGS | C14-gNccGTAGSLAQNDNKPK | 2 |
| AT1G50700.1 | Calcium-dependent protein kinase 33 (CPK33) | IV.1.b | X | X | MGNCLAKKYG | C14-gNCLAK | 2 | |
| AT1G64850.1 | Calcium-binding EF hand family protein (Ca EF) | II.2 | X | X | MGQVFNKLRG | C14-gQVFNK | 2 | |
| AT1G49580.1 | CDPK-related kinase 8 (CRK8) | IV.1.b | X | X | MGGCTSKPST | C14-gGCTSK | 2 | |
| AT3G50530.1 | CDPK-related kinase 5 (CRK5) | IV.1.b | X | X | X | MGLCTSKPNS | C14-gLcTSKPNSSNSDQTPAR | 2 |
| AT5G24430.1 | CDPK-related kinase 4 (CRK4) | IV.3.a | X | X | MGHCYSRNIS | C14-gHCYSR | 2 | |
| AT2G46700.1 | CDPK-related kinase 3 (CRK3) | IV.1.b | X | X | MGQCYGKVNQ | C14-gQCYGK | 2 | |
| AT4G39320.1 | Unknown function | IV.1.b | X | X | MGNCALKPKV | C14-gNcAIKPK | 2 | |
| AT3G23280.1 | RING E3 Ligase (XBAT35) | X | X | MGQQQSKGEL | C14-gQQQSK | 2 | ||
| AT1G14060.1 | Unknown function | II.1 | X | X | MGITSSTDPK | C14-gITSSTDPK | 2 | |
| AT5G07910.1 | Leucine-rich repeat family protein (LRR-like) | IV.1.b | X | X | X | MGCCASNTAG | C14-gccASNTAGGSK | 2 |
| AT3G27930.1 | Unknown function | I.2 | X | X | X | MGNALVKKEP | C14-gNAIVK | 2 |
| AT5G66290.1 | Unknown function | III.1.a | X | X | MGASDSTLLG | C14-gASDSTLLGSQENR | 2 | |
| AT5G39590.1 | Unknown function | III.1.b | X | X | X | MGASSSTDDK | C14-gASSSTDDKESSEK | 2 |
| AT1G73390.1 | Unknown function | IV.1.b | X | X | MGCFASRPND | C14-gcFASRPNDTGGNR | 2 | |
| AT1G52320.2 | Unknown function | IV.1.b | X | X | MGCAQSKIEN | C14-gCAQSK | 2 | |
| AT1G32190.1 | α/β-Hydrolases superfamily | IV.3.b | X | X | X | MGCMFSHLAA | C14-gcmFSHLAAK | 2 |
| AT5G14390.1 | α/β-Hydrolases superfamily | IV.1.b | X | MGGVTSSVAA | C14-gGVTSSVAAK | 2 | ||
| AT3G01690.1 | α/β-Hydrolases superfamily | X | MGGVTSSVAA | C14-gGVTSSVAAK | 2 | |||
| AT1G66900.1 | α/β-Hydrolases superfamily | X | MGGVTSSIAA | C14-gGVTSSIAAK | 2 | |||
| AT4G24760.1 | α/β-Hydrolases superfamily | X | MGGVTSSMAA | C14-gGVTSSmAAK | 2 | |||
| AT3G30380.1 | α/β-Hydrolases superfamily | X | MGAVTSSMAA | C14-gAVTSSmAAK | 2 | |||
| AT1G13610.1 | α/β-Hydrolases superfamily | X | MGIATSTMAA | C14-gIATSTmAAK | 2 | |||
| AT5G23395.1 | Cox19-like CHCH family protein (MIA40) | X | MGQAQSDENS | C14-gQAQSDENSIPTTTTTNTPPPSANSPR | 2 | |||
| AT3G27210.1 | Unknown function | X | X | MGSSSSSSLN | C14-gSSSSSSLNNSPIR | 2 | ||
| AT1G04200.1 | Unknown function | X | X | MGGVPSTPRK | C14-gGVPSTPR | 2 | ||
| AT2G44260.1 | Unknown function (DUF946 domain) | X | X | X | MGNCLSTSDP | C14-gNcLSTSDPSHEDVSK | 2 | |
| AT1G53400.1 | Unknown function (Ubiquitin domain) | X | X | X | MGCAGSTQSQ | C14-gcAGSTQSQADGSVK | 2 | |
a
Protein accession number (TAIR v.10). Proteins identified ambiguously are indicated as protein groups.
b
Protein name based on PPDB annotation.
c
Cluster number.
d
Predicted myristoylome (Martinez et al., 2008).
e
Identification of MYRed peptide by LTQ-Orbitrap (Velos) (left column) and by Triple-TOF (right column).
f
N-terminal protein sequence (10 amino acids).
g
Sequence of identified N-terminal MYRed peptides. Identified modified amino acid is indicated in lowercase. Myristoyl moiety is indicated as C14.
h
Position of the MYRed Gly.