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. 2018 Feb 15;30(3):528–542. doi: 10.1105/tpc.17.00238

Figure 4.

Figure 4.

Molecular Impact of the Trojan Horse on Somatic Cell Fate in Zmmac1-1 Anthers.

(A) Schematic comparison of cell fate specification progression in W23 fertile and Zmmac1-1 anthers up to 800 μm. Lighter hues indicate fate specification and darker hues mature cell types.

(B) to (G) RNA sequencing analysis of U. maydis genes or maize genes in fertile anthers infected with SG200 and Zmmac1-1 anthers infected with SG200 or SG200PUmpit2::SpUmpit2-Zmmac1-mCherry-Ha (SG200Zmmac1).

(B) Principal component analysis of U. maydis gene expression revealed differential infections between samples. Samples with comparable infection are highlighted by a dashed circle and used for the analysis shown in (C) to (G).

(C) to (G) Expression (normalized abundances) comparison by linear regression of pluripotency markers ([C]; Zhang et al., 2014), somatic niche markers ([D]; Kelliher and Walbot, 2014), photosynthesis-associated genes ([E]; Zhang et al., 2014; Murphy et al., 2015), archesporial marker genes ([F]; Kelliher and Walbot, 2014), and meiosis-associated genes ([G]; Kelliher and Walbot, 2014).