Figure 6.

Bleomycin and Cisplatin Treatments Result in Dose-Dependent Growth Impairments.

ΔPpRecQ4 plants are affected most severely while the expression of AtRecQ4A in ΔPpRecQ4 background leads to an intermediate phenotype compared with wild-type and ΔPpRecQ6 plants. Protonema of the wild type, ΔPpRecQ4-1 (ΔRecQ4), ΔPpRecQ6-1 (ΔRecQ6), and AtRecQ4A_ΔPpRecQ4-1#2 (AtRecQ4A_ΔRecQ4) was subjected to treatment with 50 µg/mL (middle) or 200 µg/mL (bottom) bleomycin (1.5–2.0 units/mg) or incubated in Knop medium as a control (top) for 24 h (A) or 0.33 mM (middle), 0.66 mM (bottom) cisplatin, or Knop medium with 0.66% DMSO (corresponding amount of DMSO to the treatment with 0.66 mM cisplatin; top) for 48 h (B). After washing with minimal medium, spot inocula were transferred to solid medium to regenerate for 4 weeks. Bars = 1 mm.