CaM2 Silencing Compromises the PSK-Induced Immunity and IAA Accumulation.
(A) Representative chlorophyll fluorescence imaging of ΦPSII as affected by CaM2 silencing and application of PSK and NAA. Tomato CaM2-silenced plants were treated with 10 µM PSK or 50 nM NAA 12 h before B. cinerea inoculation, and leaf samples were collected at 2 dpi. Bar = 1 cm.
(B) Quantification of ΦPSII at 2 dpi.
(C) Relative B. cinerea actin transcript abundance in infected leaves at 1 dpi.
(D) Changes in IAA content in CaM2-silenced tomato plants at 0.5 dpi, as affected by exogenous PSK and NAA application.
The results in (B) to (D) are presented as mean values ± sd; n = 3 leaves from different plants in (B); n = 3 independent pooled samples with each from two plants in (C) and (D). Different letters indicate significant differences between treatments (P < 0.05, Tukey’s test). The above experiments were repeated three times with similar results.