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AtLEGγ Can Be Stored Efficiently at High Concentrations.
(A) The specific activity of zymogenic AtLEGγ (proAtLEGγ) at pH 4.0 is inversely correlated to its concentration, indicating a strongly autoinhibitory effect of dimerization. Error bars represent standard deviations of triplicate measurements.
(B) SDS-PAGE shows the concentration-dependent autoactivation of proAtLEGγ at a low (0.25 mg/mL; black) and high concentration (2.45 mg/mL; blue).
