Figure 7.

ARF8.4 Directly Binds MYB26 Promoter.

(A) Schematic diagram of putative ARF binding sites in the MYB26 promoter (900 bp from the transcription start site). The upper black lines indicate fragments amplified in ChIP-qPCR assays.

(B) Chromatin immunoprecipitation analysis of ARF8.4 and ARF8.2 binding to the MYB26 promoter. A very high fold enrichment was observed in regions A and B in ARF8.4-overexpressing inflorescences and a very low fold enrichment was observed in all three regions in ARF8.2-overexpressing inflorescences. ChIP-qPCR values are means ± se of nine data points obtained from three biological replicates that were each analyzed in triplicate. Biological replicates were obtained by pooling inflorescences isolated from 50 independently grown plants for each genotype. Asterisks indicate a significant difference from the -Ab control value: *P < 0.05, **P < 0.01, and ***P < 0.001. Ab, antibody.