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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Chem Rev. 2017 Jul 19;117(15):10377–10402. doi: 10.1021/acs.chemrev.7b00100

Figure 5.

Figure 5

In vivo peptide-selection strategy using an AAV2 library. First, an AAV2 library is injected into the animal model. The tissue of interest is then harvested after 6 days. Library insert fragments from the AAV2 library in the DNA are isolated and amplified using a polymerase chain reaction. The insert DNA is then cloned into plasmids and transfected into 293T cells to produce a secondary AAV2 library for the next round of selection (typically 2–5 rounds).105 Adapted with permission from ref105. Copyright 2016 Elsevier.