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. 2018 Apr 4;17:1533034618765221. doi: 10.1177/1533034618765221

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Rescue of FOXM1 in miR-212-expressing cells partially restored cell migration. A, Western blot analysis of FOXM1 expression and related proteins in HCCLM3 cells transfected with empty vector as control, pCDNA3-FOXM1 or pCDNA3-FOXM1, and miR-212. Lower panel: Densitometric analysis of immunodetected bands. Data were normalized using GAPDH expression. B, Transwell analysis for FOXM3 and miR-212 in HCCLM3 cells transfected with empty vector, pCDNA3-FOXM1 or pCDNA3-FOXM1 and miR-212. The histograms represent the migrated cells ± SD from 3 independent experiments after normalization. C, Wound healing analysis for FOXM3 and miR-212 in HCCLM3 cells transfected with empty vector pCDNA3-FOXM1 or pCDNA3-FOXM1 and miR-212. The histograms represent the migration distance ± SD from 3 independent experiments after normalization. FOXM1 forkhead box M1; HCC, hepatocellular carcinoma.