Figure 6.

miR-212 impairs the growth of hepatocellular carcinoma xenografts in vivo. A, Efficient infection of HCCLM3 cells with lentivirus shown by fluorescence microscopy. The black and white pictures show the cells in the same field under normal white light. B, The tumor weights were measured and calculated. C, Mice were killed and photographed 24 days after injection of HCCLM3 cells. D, HCCLM3 cells infected with lentivirus LV-miR-212 or LV-miR-con were injected subcutaneously into nude mice. Tumor size measurements began on the sixth day and occurred every other day for 24 consecutive days. The recorded data were used to generate tumor growth curves, as shown. Data are presented as the mean (SD) tumor volume. E, Mice were injected with tumor cells and killed after 24 days. Livers were dissected and fixed in formalin, embedded in paraffin, and sectioned for hematoxylin and eosin staining. *P < .05. HCC indicates hepatocellular carcinoma.